How to avoid the rabbit Fab's toxicity in the process of rabbit antibody product

AveryLee

How to avoid the rabbit Fab's toxicity in the process of rabbit antibody production ? There are two approved approaches to avoid toxicity, insolubility and low expression problems of rabbit Fab in bacterial cells. The first is to make rabbit/human chimeric Fab libraries in which rabbit variable domains (Vλ, Vk, and VH) are linked to human constant domains (Ck and CH1). This approach has been well demonstrated by the work done by Drs. Carlos F. Barbas III and Christoph Rader. The other approach is to express the selected rabbit Fab (or scFv) antibodies in yeast Pichia pastoris, relying on yeast display technology.