Micro-propogation( tissue culture )

Obersliutenant

sekadar nak bentangkan perbincangan mengenai  hot topik biology

kelebihan micro-propogation--> mampu menghasilkan anak-anak pokok klon yg mempunyai ciri2 seumpama pokok induk. Kelebihanya juga adalah mana2 bahagian boley diambil dari pokok induk dan dibesarkan dlm tabung uji yang steril sehingga besar. Anak2 pokok tak akan dipengaruhi gen-gen resesif vs dominan dimana sebaliknya kualiti klon pokok2 dapat di control dan dihasilkan secara besar-besaran. Adakah sesiapa yg boleh explain dengan lebih terperinci bagaimana  membuat 'micro-propogation' dan tissue culture?
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[ Last edited by  Obersliutenant at 4-10-2007 02:28 AM ]

ischae

use agrobacterium to transfer desired genes..of course good genes

then select for transgenic ones..which is being transferred by agrobacterium

it doesnt mean xde lansung dominant n resessive genes

just tht by using this method, certain genes could be inhibited

u want me explain the protocol or what?

Obersliutenant

it woud be helpful if you can explain me the process... im hunting down a do-it-yourself formulae for this ...Thanks

tarabas1976

he he he the probability of obtaining transgenic plant is 1 every 1000 try.

mbhcsf

logiknya this is all stemmed from mitotic division lah since any tissue from any part, kan? so does it involves "only" the somatic cells? I am just thinking.

tarabas1976

actually you can take not only the somatic cells, but any cells which has a totipotency...such as nodes, internodes, young leaf. You can even use an embyogenic cells (such as zygotic embyo). From any part that youi used you can make it embyogenic and thus induce somatic embryogenesis using manipulation of 2,4-D, malt extract and other PGr.

For beginners, micropropagation is easy, you can try this book for beginners part

In vitro culture of higher plants by R.L.M. Pierik.

Feno

wondering the media property used for tissue culture?